Recombinant Matrix Metalloproteinase 12 (MMP12) Oryctolagus cuniculus (Rabbit) Recombinant protein

MME; HME; ME; Macrophage Metalloelastase; Macrophage Elastase

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Molecular Mass 22.5kDa, Accurate 22kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal286~Cys464 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point9.5
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  • Recombinant Matrix Metalloproteinase 12 (MMP12) Packages (Simulation)
  • Recombinant Matrix Metalloproteinase 12 (MMP12) Packages (Simulation)
  • RPA402Rb01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repairPubmed: 23619615
  • Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Inspiratory resistive breathing induces MMP-9 and MMP-12 expression in the lungPubmed:25595645
  • Protective effects of Aloe sterols against UVB‐induced photoaging in hairless micepubmed:27995657
  • Яковлева Наталья Юрьевна
  • Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
  • Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient micePubmed:35031603

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