Recombinant Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Homo sapiens (Human) Recombinant protein

PRKAa1; AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Cytoplasm
  • Molecular Mass 29.6kDa, Accurate 31kDa(Analysis of differences refer to the manual)
  • Residues & TagsIle28~Lys257 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point8.5
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  • Recombinant Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Packages (Simulation)
  • Recombinant Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Packages (Simulation)
  • Recombinant Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) Figure. Gene Sequencing (Extract)
  • RPA679Hu01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletionPubmed: 31863073

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