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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)
LEKTI; LETKI; NETS; NS; VAKTI; Lymphoepithelial Kazal-Type-Related Inhibitor
Organism species Homo sapiens (Human)
Product No.RPH145Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass35.7kDa
Predicted isoelectric point8.8
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location Secreted
ResiduesGly699~Ser976 (Accession # Q9NQ38) with two N-terminal Tags, His-tag and T7-tag
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GG GNTQDECAEY REQMKNGRLS CTRESDPVRD ADGKSYNNQC TMCKAKLERE AERKNEYSRS RSNGTGSESG KDTCDEFRSQ MKNGKLICTR ESDPVRGPDG KTHGNKCTMC KEKLEREAAE KKKKEDEDRS NTGERSNTGE RSNDKEDLCR EFRSMQRNGK LICTRENNPV RGPYGKMHIN KCAMCQSIFD REANERKKKD EEKSSSKPSN NAKDECSEFR NYIRNNELIC PRENDPVHGA DGKFYTNKCY MCRAVFLTEA LERAKLQEKP SHVRAS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAH145Hu21Monoclonal Antibody to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)WB, ICC, IHC-P, IHC-F, ELISA
PAH145Hu01Polyclonal Antibody to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)WB, ICC, IHC-P, IHC-F, ELISA
RPH145Hu01Recombinant Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)SDS-PAGE; WB; ELISA; IP.
SEH145HuELISA Kit for Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)ELISA