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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1
Organism species Homo sapiens (Human)
Product No.RPG945Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass28.2kDa
Predicted isoelectric point6.1
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor.
ResiduesGln2~Ala223 (Accession # P09936) with two N-terminal Tags, His-tag and T7-tag.
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag., its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- QLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAG945Hu21Monoclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)WB, ICC, IHC-P, IHC-F, ELISA
PAG945Hu01Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)WB, ICC, IHC-P, IHC-F, ELISA
RPG945Hu01Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)SDS-PAGE; WB; ELISA; IP.
SEG945HuELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)ELISA