ELISA Kit DIY Materials for Caspase 1 (CASP1) Homo sapiens (Human) Assay Kit DIY Materials

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates , cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.118ng/mL
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  • ELISA Kit DIY Materials for Caspase 1 (CASP1) Packages (Simulation)
  • ELISA Kit DIY Materials for Caspase 1 (CASP1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Caspase 1 (CASP1). No significant cross-reactivity or interference between Caspase 1 (CASP1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response.pubmed:28704412
  • NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitinationPubmed:29386291
  • Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?Pubmed:29665864
  • A role for the inflammasome in spontaneous labor at termPubmed:27852921
  • CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSESPubmed: 29386291
  • NLRP3 inflammasome expression in lipopolysaccharide-induced otitis mediaPubmed: 30676848
  • Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss womenPubmed: 31148259
  • Serum Caspase-1 levels in women with polycystic ovary syndromePubmed: 32127139
  • Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
  • ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES
  • CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER
  • The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation34560082
  • The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice34632484
  • TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion InjuryPubmed:35041191
  • Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network DispersionPubmed:35062292

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