ELISA Kit DIY Materials for Complement 1q (C1q) Homo sapiens (Human) Assay Kit DIY Materials

C1-q; Autoantigen Clq

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range78-5,000pg/mL
  • Applicable Sensitivity30pg/mL
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  • ELISA Kit DIY Materials for Complement 1q (C1q) Packages (Simulation)
  • ELISA Kit DIY Materials for Complement 1q (C1q) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Complement 1q (C1q).No significant cross-reactivity or interference between Complement 1q (C1q) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activityPubmed: 23085472
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • The role of complement components C1q, MBL and C1 inhibitor in pathogenesis of endometriosisPubmed:29572748
  • Intrathecal complement activation by the classical pathway in tick-borne encephalitisPubmed: 30850976
  • Complement activation profile of patients with primary focal segmental glomerulosclerosisPubmed: 32569286
  • Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia33248023
  • Plasma and urine complement activation products in light chain and heavy chain deposition disease
  • Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity34280490
  • Elevated Serum Complement C1q Levels After Traumatic Brain Injury and Its Association with Poor PrognosisPubmed:35035218

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