ELISA Kit DIY Materials for Corticosteroid Binding Globulin (CBG)

SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
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Overview

Properties

Product No.KSB226Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryEndocrinology

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable Sampleserum, plasma and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range3.12-200ng/mL
Applicable Sensitivity1.25ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Corticosteroid Binding Globulin (CBG). No significant cross-reactivity or interference between Corticosteroid Binding Globulin (CBG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

Increment services

Citations

The Regulation of Rasd1 Expression by Glucocorticoids and Prolactin Controls Peripartum Maternal Insulin SecretionEndjournals: Source
Modulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria DietPubMed: PMC3579843
Differences in colocalization of corticosteroid-binding globulin and glucocorticoid receptor immunoreactivity in the rat brainScienceDirect: S0940960212001628
Adrenocortical control in the neonatal rat: ACTH‐and cAMP‐independent corticosterone production during hypoxiaPubmed: 24303136
Adrenocorticotropic Hormone But Not High-Density Lipoprotein Cholesterol or Salivary Cortisol Was a Predictor of Adrenal Insufficiency in Patients With Septic Shock.Pubmed: 24667620
Greater glucocorticoid receptor activation in hippocampus of aged rats sensitizes microgliaPubmed:25559333
Novel p53 target genes secreted by the liver are involved in non-cell-autonomous reCavia (Guinea pig )lationPubmed:26358154
Impact of Season and Oral Contraceptive use on Cortisol Levelsin Physically Active Womenexercise-sports-orthopedics:exercise-sports-orthop
Impact of Season and Oral Contraceptive use on Cortisol Levels in Physically Active Womendiva2:1047030
Impaired glucocorticoid-mediated HPA axis negative feedback induced by juvenile social isolation in male ratsPubmed:29407214
The impact of early-life stress on corticosteroid carrier protein levels and 11β-hydroxysteroid dehydrogenase 1 expression in adolescent rats