ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78) Homo sapiens (Human) Assay Kit DIY Materials

CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.055ng/mL
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  • ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78) Packages (Simulation)
  • ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Epithelial Neutrophil Activating Peptide 78 (ENA78).No significant cross-reactivity or interference between Epithelial Neutrophil Activating Peptide 78 (ENA78) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis.Pubmed:26862043
  • Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitispubmed:26862043
  • The clinical significance of CXCL5 in non-small cell lung cancer.pubmed:29200871
  • Activated CXCL5-CXCR2 axis promotes the migration, invasion and EMT of papillary thyroid carcinoma cells via modulation of β-catenin pathwayPubmed:29471001
  • Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 …Pubmed: 30404567
  • Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from …Pubmed: 30634975
  • Interleukin17–CXCR2 axis facilitates breast cancer progression by up-regulating neutrophil recruitmentPubmed: 31654638
  • Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy34820587
  • Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinomaPubmed:35141005

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