ELISA Kit DIY Materials for Fatty Acid Binding Protein 1 (FABP1) Homo sapiens (Human) Assay Kit DIY Materials

FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; Fatty Acid Binding Protein 1, Liver

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.063ng/mL
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  • ELISA Kit DIY Materials for Fatty Acid Binding Protein 1 (FABP1) Packages (Simulation)
  • ELISA Kit DIY Materials for Fatty Acid Binding Protein 1 (FABP1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 1, Liver (FABP1).No significant cross-reactivity or interference between Fatty Acid Binding Protein 1, Liver (FABP1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart DiseasePubmed:25120320
  • Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusionPubmed:25555715
  • Controlled Rewarming after Hypothermia: Adding a New Principle to Renal PreservationPubMed: 26053383
  • The role of serum I-FABP concentration in assessment of small intestine mucosa among HIV-infected patientsContent: Early
  • Kidney transplantation after oxygenated machine perfusion preservation with Custodiol‐N solutionPubMed: 25882869
  • Prediction of renal function upon reperfusion by ex situ controlled oxygenated rewarmingpubmed:27718228
  • The Importance of Liver-Fatty Acid Binding Protein in Diagnosis of Liver Damage in Patients with Acute Hepatitispubmed:28571184
  • Plasma Free Fatty Acids and their Binding Proteins in Preterm InfantsPubmed:30045009
  • Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
  • Circulating fatty acid-binding protein 1 (FABP1) and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitusPubmed: 32038102
  • FABP1 and FABP2 as markers of diabetic nephropathyPubmed: 32922200
  • Profile of Intestinal Barrier Functional Markers in Italian Patients with Diarrhea-Predominant IBS: Preliminary Data from a Low-FODMAPs diet Trial
  • The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males34071378
  • TCA Cycle and Fatty Acids Oxidation Reflect Early Cardiorenal Damage in Normoalbuminuric Subjects with Controlled Hypertension34356333
  • Association of plasma fatty acid-binding protein 3 with estimated glomerular filtration rate in patients with type 2 diabetes mellitus34975301

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