ELISA Kit DIY Materials for Fibrinogen Like Protein 2 (FGL2) Mus musculus (Mouse) Assay Kit DIY Materials

T49; PT49; Fibroleukin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SamplePlasma.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.137ng/mL
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  • ELISA Kit DIY Materials for Fibrinogen Like Protein 2 (FGL2) Packages (Simulation)
  • ELISA Kit DIY Materials for Fibrinogen Like Protein 2 (FGL2) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 2 (FGL2). No significant cross-reactivity or interference between Fibrinogen Like Protein 2 (FGL2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Expression of B and T lymphocyte attenuator (BTLA) in macrophages contributes to the fulminant hepatitis caused by murine hepatitis virus strain-3PubMed: 22637698
  • Plasma fibrinogen-like protein 2 levels in patients with non-alcoholic fatty liver disease.PubMed: 22024080
  • Programmed Death (PD)-1-Deficient Mice Are Extremely Sensitive to Murine Hepatitis Virus Strain-3 (MHV-3) InfectionPubMed: PMC3131267
  • Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection.Pubmed: 24286726
  • Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infectionPubmed:24286726
  • VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolismpubmed:29109438
  • Soluble fibrinogen‐like protein 2 ameliorates acute rejection of liver transplantation in rat via inducing Kupffer cells M2 polarizationPubmed:29749104
  • Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseasesPubmed: 30419833
  • Tandem mass tag©\based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device33354778

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