ELISA Kit DIY Materials for Growth Differentiation Factor 15 (GDF15) Homo sapiens (Human) Assay Kit DIY Materials

PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.069ng/mL
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  • ELISA Kit DIY Materials for Growth Differentiation Factor 15 (GDF15) Packages (Simulation)
  • ELISA Kit DIY Materials for Growth Differentiation Factor 15 (GDF15) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Growth Differentiation Factor 15 (GDF15).No significant cross-reactivity or interference between Growth Differentiation Factor 15 (GDF15) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Growth Differentiation Factor 15 and Coronary Collateral FormationPubMed: 20014173
  • Elevated levels of GDF-15 is associated with increased angiotensin II in hypertensive patients with Type 2 diabetespme-2016-0030
  • Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
  • Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
  • Serum NT©\proBNP levels are associated with cognitive functions in hemodialysis patients33423325
  • Is GDF-15 level associated with gestational diabetes mellitus and adverse perinatal outcomes?33678319
  • Changes in Plasma Growth Differentiation Factor-15 After Laparoscopic Sleeve Gastrectomy in Morbidly Obese Patients: A Prospective Study33880052
  • Predictive value of growth differentiation factor-15 in patients with myocardial infarction
  • High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637
  • Small extracellular vesicle-mediated targeting of hypothalamic AMPKα1 corrects obesity through BAT activation34675439
  • Growth Differentiation Factor 15 is Related with Left Ventricular Recovery in Patients with ST-Elevation Myocardial Infarction after Successful Reperfusion by …34584380
  • The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in ratPubmed:35029766
  • Plasma growth differentiation factor‐15 in patients with “lone” atrial fibrillationPubmed:35334497

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