ELISA Kit DIY Materials for Interleukin 1 Receptor Type I (IL1R1) Mus musculus (Mouse) Assay Kit DIY Materials

CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.25-2000pg/mL
  • Applicable Sensitivity14.8pg/mL
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  • ELISA Kit DIY Materials for Interleukin 1 Receptor Type I (IL1R1) Packages (Simulation)
  • ELISA Kit DIY Materials for Interleukin 1 Receptor Type I (IL1R1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Type I (IL1R1). No significant cross-reactivity or interference between Interleukin 1 Receptor Type I (IL1R1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • In vivo extravasation induces expression of IL-1R1 in human neutrophilsWiley: source
  • The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective EndocarditisPlosone: 0031490
  • Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel diseasePubMed: 22267087
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • IL1R1 基因多态性与儿童哮喘的相关性article:13877
  • IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathwaysPubmed: 33107399
  • Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in ratsPubmed:35643766

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