ELISA Kit DIY Materials for Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,361.00 US$ 2,268.00 US$ 4,082.00 US$ 7,938.00 US$ 13,608.00
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Overview

Properties

Product No.KSA780Bo01
Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryTumor immunity Infection immunity

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable Sampleserum, plasma, tissue homogenates, cell lysates, saliva, milk,cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range1.56-100ng/mL
Applicable Sensitivity0.62ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Lactoferrin (LTF).No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reactionScienceDirect: S1357272511002524
Changes in composition of colostrum of Egyptian buffaloes and Holstein cowsBioMed: 17466148
p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during PregnancyPubmed:25505239
Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4Pubmed:26974438
Salivary lactoferrin is transferred into the brain via the sublingual routepubmed:28351211
Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cellsPubmed:29627483
TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889
Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093
Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung InjuryPubmed:35625931