ELISA Kit DIY Materials for Sirtuin 1 (SIRT1) Homo sapiens (Human) Assay Kit DIY Materials

SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.28ng/mL
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  • ELISA Kit DIY Materials for Sirtuin 1 (SIRT1) Packages (Simulation)
  • ELISA Kit DIY Materials for Sirtuin 1 (SIRT1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Sirtuin 1 (SIRT1).No significant cross-reactivity or interference between Sirtuin 1 (SIRT1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular DiseasesPlos: Source
  • A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in RatsPubmed:25501750
  • Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive ImpairmentPubmed:25589731
  • Increases in peripheral SIRT1: A new biological characteristic of asthmaPubMed: 26040995
  • A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:PubMed: 25785999
  • Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.pubmed:27522360
  • The potential curative effect of rebamipide in hepatic ischemia/reperfusion injurypubmed:28361247
  • A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery diseasepubmed:28043667
  • Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trialpubmed:28029409
  • Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han populationpubmed:29189974
  • Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)pubmed:28974454
  • Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunctionpubmed:28919305
  • Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …Pubmed:30037068
  • Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementiaPubmed:30071285
  • The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
  • Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical studyPubmed: 30556451
  • Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic …
  • Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária
  • Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility
  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of …Pubmed: 30956976
  • Vasohibin-1 in T2DM with different UACR
  • The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance HemodialysisPubmed: 32214805
  • Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factorPubmed: 33045669
  • Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте
  • Dietary buckwheat enhances sirtuin1 without calorie restriction
  • Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study33447019
  • The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡­33396247
  • Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease33864594
  • Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡­34052296
  • Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories34174262
  • Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats34763933
  • Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease34774622
  • Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient RatsPubmed:35163435
  • Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?Pubmed:35683374
  • OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress

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