ELISA Kit DIY Materials for Thymidine Phosphorylase (TP)

TYMP; PDECGF; ECGF1; MNGIE; TP; HPD-ECGF; TdRPase; Platelet-Derived Endothelial Cell Growth Factor; Gliostatin

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
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Overview

Properties

Product No.KSA948Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryEnzyme & Kinase Tumor immunity

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids。
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range1.56-100ng/mL
Applicable Sensitivity0.56ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Thymidine Phosphorylase (TP). No significant cross-reactivity or interference between Thymidine Phosphorylase (TP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosisPubmed: 24188612
Liver as a source for thymidine phosphorylase replacement in mitochondrial neurogastrointestinal encephalomyopathyPubmed:24802030
Thymidine phosphorylase expression is associated with time to progression in patients with metastatic colorectal cancerPubmed:24936150
Capecitabine reverses tumor escape from anti-VEGF through the eliminating CD11bhigh/Gr1high myeloid cellsPubmed:29707135
Prognostic significance of serum translocator protein in patients with traumatic brain injuryPubmed: 30385279
Usefulness of postoperative serum translocator protein as a predictive marker for delirium after breast cancer surgery in elderly womenPubmed: 32529881
Targeting thymidine phosphorylase as a potential therapy for bone loss associated periprosthetic osteolysis