ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP) Rattus norvegicus (Rat) Assay Kit DIY Materials

GC; VDBG; VDBP; Group-Specific Component; Gc-globulin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200ng/mL
  • Applicable Sensitivity1.42ng/mL
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  • ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP) Packages (Simulation)
  • ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP). No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patientsPubMed: 23101585
  • Increased Circulating Levels of Vitamin D Binding Protein in MS PatientsPubmed:25590278
  • Vitamin D-binding protein and free vitamin D concentrations in acromegalyPubMed: 26547217
  • Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
  • 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients.pubmed:28085957
  • Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
  • 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients10.1371/journal.pone.0170323
  • Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …Pubmed:30037068
  • Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …Pubmed: 30553018
  • Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children?34013709
  • Leukocyte telomere length as a compensatory mechanism in vitamin D metabolismPubmed:35202418

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