ELISA Kit DIY Materials for 25-Hydroxyvitamin D3 (HVD3)

25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
Quantity

Add to Cart Distributors

Overview

Properties

Product No.KSA915Ge11
Organism SpeciesPan-species (General) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryReproductive science Genetic science Nutrition metabolism Bone metabolism

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma and other biological fluids.
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range2.47-200ng/mL
Applicable Sensitivity0.91ng/mL

Share your citation Upload your experimental result Review Leave a message

Sign into your account

Email *

Password *

Verification code*

Create a new account

Share a new citation as an author

Title *

Journal

Link*

Upload your experimental result

Application*

Experimental result*

Review

Rating

Serial No* Please attach serial No. on instruction manual

Contact us
Please fill in the blank.

Name*

Organization

Address

E-mail address*

Telephone

Inquiry*

Verification code* CheckCode

Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of 25-Hydroxyvitamin D3 (HVD3). No significant cross-reactivity or interference between 25-Hydroxyvitamin D3 (HVD3) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Giveaways

ELISA / CLIA Experiment Service

Increment services

Citations

Decreased serum LL-37 and vitamin D3 levels in atopic dermatitis: relationship between IL-31 and oncostatin MWiley: source
成人哮喘血清维生素D 水平与肺功能相关因素分析Jwk_Tjyy: Cn
Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
Chronic maternal calcium and 25-hydroxyvitamin D deficiency in Wistar rats programs abnormal hepatic gene expression leading to hepatic steatosis in female offspring.pubmed:28219837
Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Levelpubmed:28064234
Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis.pubmed:29094019
Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar RatsPubmed:29850540
Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseasesPubmed: 31407857
Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in …Pubmed: 33065276
The Effects of Follicular Fluid 25 (OH) D Concentration on Intrafollicular Estradiol Level, Oocyte Quality, and Fertilization Rate in Women Who Underwent IVF Program