ELISA Kit DIY Materials for Alpha-Tocopherol (TCPa)

VE; Vitamin E; α-TCP; α-Tocopherol

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,433.00 US$ 2,388.00 US$ 4,298.00 US$ 8,358.00 US$ 14,328.00
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Overview

Properties

Product No.KSA922Ge11
Organism SpeciesPan-species (General) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryMetabolic pathway Nutrition metabolism

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma and other biological fluids
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range1.23-100ug/mL
Applicable Sensitivity0.46ug/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Tocopherol (TCPa). No significant cross-reactivity or interference between Alpha-Tocopherol (TCPa) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Evaluation of enzymatic and non- enzymatic antioxidant status in seminal plasma of Iraqi Infertile MenCom:Source
Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats.PubMed: 25903950
The potential effects of antioxidant feed additives in mitigating the adverse effects of cornnaturally contaminated with Fusarium mycotoxins on antioxidant systems in the intestinal mucosa, plasma, and liver in weaned pigs.Pubmed:27021614
Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genespubmed:27816814
Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responsespubmed:29102903
Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolismPubmed: 30973911
Prooxidant effect of α-tocopherol in pancreatic tumors.
Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study