ELISA Kit DIY Materials for Betacellulin (bTC)
Probetacellulin
- UOM
- FOB US$ 979.00 US$ 1,632.00 US$ 2,938.00 US$ 5,712.00 US$ 9,792.00
- Quantity
Overview
Properties
- Product No.KSA112Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only - Downloadn/a
- CategoryCytokineTumor immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.62-1000pg/mL
- Applicable Sensitivity6.4pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Betacellulin (bTC). No significant cross-reactivity or interference between Betacellulin (bTC) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicinePubmed: 33212364
- Compromised Cumulus-Oocyte Complex Matrix Organization and Expansion in Women with PCOS34748173