ELISA Kit DIY Materials for Caveolin 1 (CAV1) Homo sapiens (Human) Assay Kit DIY Materials

VIP21; Caveolae Protein 22kDa

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.24-15ng/mL
  • Applicable Sensitivity0.11ng/mL
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  • ELISA Kit DIY Materials for Caveolin 1 (CAV1) Packages (Simulation)
  • ELISA Kit DIY Materials for Caveolin 1 (CAV1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Caveolin 1 (CAV1). No significant cross-reactivity or interference between Caveolin 1 (CAV1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Novel prostate cancer tumour markers in a cell line modelMendelu: source
  • Caveolin-1 as a potential high-risk prostate cancer biomarkerPubMed: 22159333
  • Increased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese menPubmed: 23645736
  • Decrased sputum caveolin-1 is associated with systemic sclerosis related lung diseasePubmed:24751454
  • Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer ProgressionPubMed: 26543085
  • Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarctionPubmed:26775120
  • Increased caveolin-1 in intervertebral disc degeneration facilitates repairPubmed:26939667
  • Lower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic StrokePubmed:27119011
  • Caveolin-1 Promotes the Imbalance of Th17/Treg in Patients with Chronic Obstructive PulmonaryDisease.pubmed:27613621
  • Swim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis MicePubmed:29849903
  • Endothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced …Pubmed:29665152
  • The CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic …
  • SIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR¦Ã34077730
  • Cav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA?¡­
  • 5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 ProteinPubmed:35566232

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