ELISA Kit DIY Materials for Ethinylestradiol (EE)
Ethinyl Estradiol ; Ethynyl Estradiol; Ethinyl Oestradiol
- UOM
- FOB US$ 1,469.00 US$ 2,448.00 US$ 4,406.00 US$ 8,568.00 US$ 14,688.00
- Quantity
Overview
Properties
- Product No.KSK510Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- Category
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range24.69-2000pg/mL
- Applicable Sensitivity9.34pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Ethinylestradiol (EE). No significant cross-reactivity or interference between Ethinylestradiol (EE) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Electrochemical immunosensor for ethinylestradiol using diazonium salt grafting onto silver nanoparticles-silica–graphene oxide hybridsPubMed: 26592615
- Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania150659