ELISA Kit DIY Materials for Glutamic Acid (Glu) Pan-species (General) Assay Kit DIY Materials

Glutamate

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range0.123-10ng/mL
  • Applicable Sensitivity0.055ng/mL
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  • ELISA Kit DIY Materials for Glutamic Acid (Glu) Packages (Simulation)
  • ELISA Kit DIY Materials for Glutamic Acid (Glu) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Glutamic Acid (Glu). No significant cross-reactivity or interference between Glutamic Acid (Glu) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Critical role for Annexin A7 in secondary brain injury mediated by its phosphorylation after experimental intracerebral hemorrhage in ratspubmed:29196215
  • High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
  • Neurotransmitter system aberrations in patients with drug addictionPubmed: 32804296
  • ¦Ê?opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in ¡­34036389
  • Ah-type baroreceptor neurons expressing estrogen dependent mGluR7 mediate descending inhibition of cardiac nociceptionPubmed:35436518
  • Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients …Pubmed:35566558
  • The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in RatsPubmed:35645576

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