ELISA Kit DIY Materials for Homocysteine (HCy) Pan-species (General) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range98.77-8000ng/mL
  • Applicable Sensitivity39.55ng/mL
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  • ELISA Kit DIY Materials for Homocysteine (HCy) Packages (Simulation)
  • ELISA Kit DIY Materials for Homocysteine (HCy) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Homocysteine (HCy). No significant cross-reactivity or interference between Homocysteine (HCy) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspringPubmed:29582727
  • Homocysteinemia in relation to anemia in hypothyroid patientsDoi: 10.4103/sjamf.sjamf_31_18
  • Organochlorine pesticides exposure may disturb homocysteine metabolism in pregnant womenPubmed: 31787282
  • Baicalin rescues hyperglycemia-induced neural tube defects via targeting on retinoic acid signalingPubmed: 32774702
  • Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
  • Homocysteinaemia in Heart Failure Patients in North Eastern Nigeria
  • KARD?YOVASK¨¹LER S?STEM HASTALIKLARINDA R?SK FAKT?R¨¹ OLAN H?PERHOMOS?STE?NEM?'N?N POL?K?ST?K OVER SENDROMUNDAK? YER?
  • Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, EthiopiaPubmed:35464038

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