ELISA Kit DIY Materials for Hydroxyproline (Hyp) Pan-species (General) Assay Kit DIY Materials

(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range61.7-5000ng/mL
  • Applicable Sensitivity24.1ng/mL
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  • ELISA Kit DIY Materials for Hydroxyproline (Hyp) Packages (Simulation)
  • ELISA Kit DIY Materials for Hydroxyproline (Hyp) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Hydroxyproline (Hyp). No significant cross-reactivity or interference between Hydroxyproline (Hyp) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Spleen-Derived Interleukin-10 Downregulates the Severity of High-Fat Diet-Induced Non-Alcoholic Fatty Pancreas DiseasePubMed: PMC3532347
  • Prediction of fibrosis in hepatitis C patients: assessment using hydroxyproline and oxidative stress biomarkersSpringer: Source
  • Royal jelly affects collagen crosslinking in bone of ovariectomized ratsSciencedirect:S1756464614000346
  • Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transitionPubMed: 25911951
  • Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in ratsPubMed: 26165752
  • Effects of 4% Icodextrin on Experimental Spinal Epidural Fibrosispdf:JTNEPUB_15079_online.pdf
  • Celecoxib attenuates depressive-like behavior associated with immunological liver injury inC57BL/6 mice through TNF-α and NF-κb dependent mechanisms.pubmed:27580523
  • Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with …doi.org:10.4174
  • Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbitsPubmed:29932250
  • Renin–angiotensin system inhibition ameliorates CCl-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B10.1139:cjpp-2017-0728
  • Pirfenidone suppresses bleomycin‑induced pulmonary fibrosis and periostin expression in rats10.3892:etm.2018.6378
  • Therapeutic effect of hepatocyte growth factor-overexpressing bone marrow-derived mesenchymal stem cells on CCl 4-induced hepatocirrhosis.
  • The effects of platelet‐rich‐plasma gel application to the colonic anastomosis in hyperthermic intraperitoneal chemotherapy: An experimental rat modelPubmed: 31448554
  • Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungsPubmed: 32146192
  • Is platelet-rich plasma improves the anastomotic healing in hyperthermic intraperitoneal chemotherapy with oxaliplatin: an experimental rat studyPubmed: 32051817
  • Protective Effects of Contractubex® on Stricture Formation after Experimental Corrosive Esophageal Burns in RatsPubmed: 32654881
  • Effect of Oral Channa striatus in Laparotomy Wound Healing of Malnourished Rats.
  • Handgrip Strength and Vitamin D as Predictors of Liver Fibrosis and Malnutrition in Chronic Hepatitis C Patients33884041
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
  • Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress34858986

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