ELISA Kit DIY Materials for Quinolinic Acid (QA) Pan-species (General) Assay Kit DIY Materials

QUIN; Pyridine-2,3-Dicarboxylic Acid

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range1.23-100ng/mL
  • Applicable Sensitivity0.58ng/mL
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  • ELISA Kit DIY Materials for Quinolinic Acid (QA) Packages (Simulation)
  • ELISA Kit DIY Materials for Quinolinic Acid (QA) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Quinolinic Acid (QA). No significant cross-reactivity or interference between Quinolinic Acid (QA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus.pubmed:27870896
  • Monoamine oxidase A upregulated by chronic intermittent hypoxia activates indoleamine 2, 3-dioxygenase and neurodegenerationpubmed:28599322
  • Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of RatsPubmed:29725904
  • Exercise training impacts skeletal muscle gene expression related to the kynurenine pathwayPubmed: 30649918
  • Modification of kynurenine pathway via inhibition of kynurenine hydroxylase attenuates surgical brain injury complications in a male rat modelPubmed: 31257634
  • Serotonin is the main tryptophan metabolite associated with psychiatric comorbidity in abstinent cocaine-addicted patientsPubmed: 31727978
  • Kynurenine 3-monooxygenase upregulates pluripotent genes through β-catenin and promotes triple-negative breast cancer progressionPubmed: 32268268

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