ELISA Kit DIY Materials for Retinol (Ret) Pan-species (General) Assay Kit DIY Materials

VA; Vitamin A; Aquasola

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum and plasma.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.46-10000ng/mL
  • Applicable Sensitivity52.4ng/mL
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  • ELISA Kit DIY Materials for Retinol (Ret) Packages (Simulation)
  • ELISA Kit DIY Materials for Retinol (Ret) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Retinol (Ret). No significant cross-reactivity or interference between Retinol (Ret) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effects of Vitamin A Overdose on the Immune system in ratsJpmbs: Source
  • Salivary 1, 5-Anhydroglucitol and Vitamin Levels in Relation to Caries Risk in Children
  • Oxidative stress indicators in Chinese women with PCOS and correlation with features of metabolic syndrome and dependency on lipid patternsPubmed: 31549221
  • Prooxidant effect of α-tocopherol in pancreatic tumors.
  • Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study
  • Protocol: Prevalence of specific micronutrient deficiencies in urban school going children of India aged between 6 and 16 years: study protocol for a …Pubmed:34907037
  • Prevalence of specific micronutrient deficiencies in urban school going children and adolescence of India: A multicenter cross-sectional studyPubmed:35544476
  • Retinol Depletion in COVID-19Pubmed:35664529

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