ELISA Kit DIY Materials for Tetraspanin 30Cluster of Differentiation 63 (CD63)

The Abs in the kit have high sensitivity and excellent specificity for detection of Tetraspanin 30 (TSPAN30). No significant cross-reactivity or interference between Tetraspanin 30 (TSPAN30) and analogues was observed.

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.