ELISA Kit DIY Materials for Prealbumin (PALB) Canis familiaris; Canine (Dog) Assay Kit DIY Materials

TBPA; PA; PLB; ATTR; TTR; Transthyretin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid,cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.70ng/mL
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  • ELISA Kit DIY Materials for Prealbumin (PALB) Packages (Simulation)
  • ELISA Kit DIY Materials for Prealbumin (PALB) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Transthyretin (TTR). No significant cross-reactivity or interference between Transthyretin (TTR) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Screening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic studyPubMed: 20367977
  • Circulation levels of acute phase proteins in patients with Takayasu arteritisPubMed: 20100644
  • Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2PubMed: 21130593
  • Serum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylenePubmed:24960064
  • Schwann cells contribute to neurodegeneration in transthyretin amyloidosisPubmed:25693163
  • Surface plasmon resonance assay of inhibition by pharmaceuticals for thyroxine hormone binging to transport proteinsPubMed: 26384643
  • Compositions and Methods for Diagnosing Lung Cancery2016:0077095.html
  • iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Managementpubmed:29247448
  • CD4+ T Cells Affect the Thyroid Hormone Transport at the Choroid Plexus in Mice Raised in Enriched EnvironmentPubmed: 30703773
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • Primary Cardiac Involvement in the Rare Transthyretin Ile73Val Mutation: A Case SeriesPubmed: 32393063

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