ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Mus musculus (Mouse) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.059ng/mL
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  • ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Packages (Simulation)
  • ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosisPubmed:25488180
  • Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cellsPubMed: 26547443
  • Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosisPubMed: 25488180
  • Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell culturesPubMed: 26163991
  • Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an …servlets:MCRFileNodeServlet
  • Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.pubmed:28754618
  • Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …Pubmed:29674475
  • Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal CellsPubmed:29377497
  • Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidasesPubmed: 31534019
  • Role of wnt5a in Metabolic Inflammation in HumansPubmed: 30137542
  • Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer MetastasisPubmed: 31932454
  • Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive diseasePubmed: 33188773
  • Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …Pubmed: 32491086
  • An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties34823885
  • Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort studyPubmed:34990470
  • Association between bone turnover markers and periodontitis: A population‐based cross‐sectional studyPubmed:35569034

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