ELISA Kit DIY Materials for Hemojuvelin (HJV) Homo sapiens (Human) Assay Kit DIY Materials

HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity12.7pg/mL
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  • ELISA Kit DIY Materials for Hemojuvelin (HJV) Packages (Simulation)
  • ELISA Kit DIY Materials for Hemojuvelin (HJV) Packages (Simulation)
  • KSB995Hu01.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Hemojuvelin (HJV). No significant cross-reactivity or interference between Hemojuvelin (HJV) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin ProteinPubMed: PMC3356351
  • Hepcidin Levels Are Increased in Patients with Acute Ischemic Stroke: Preliminary ReportPubMed: 25881778
  • Soluble hemojuvelin in transfused and untransfused thalassaemic subjectsdoi:10.1111
  • The secret war against counterfeit sciencepubmed:28492262
  • Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgeryPubmed:29699579
  • Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac SurgeryPubmed:29386545
  • The functional role of hemojuvelin in acute ischemic strokePubmed: 31307288
  • Oxidative damage and iron dyshomeostasis in thalassemic patients
  • Caffeine and alcohol-friends or foes of human iron stores?Pubmed:35063815
  • Urinary Biomarkers Can Predict Weaning From Acute Dialysis Therapy in Critically Ill PatientsPubmed:35311928
  • The Relationships among Plasma Fetuin-B, Thyroid Autoimmunity, and Fertilization Rate In Vitro Fertilization and Embryo TransferPubmed:35340679

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