CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3) Mus musculus (Mouse) Sandwich CLIA

SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

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  • CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3) Packages (Simulation)
  • CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3) Packages (Simulation)
  • CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Superoxide Dismutase 3, Extracellular (SOD3) and the recovery rates were calculated by comparing the measured value to the expected amount of Superoxide Dismutase 3, Extracellular (SOD3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-90 86
EDTA plasma(n=5) 81-104 85
heparin plasma(n=5) 98-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Superoxide Dismutase 3, Extracellular (SOD3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-90% 79-89% 78-90% 87-95%
EDTA plasma(n=5) 82-92% 94-101% 94-105% 83-92%
heparin plasma(n=5) 87-96% 78-92% 89-97% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Superoxide Dismutase 3, Extracellular (SOD3) level in the sample or standard.;

Citations

  • Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187
  • Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress.pubmed:28702016
  • SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2αPubmed:29422508
  • Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammationPubmed:29523072
  • Myeloperoxidase, superoxide dismutase‐3, cardiometabolic risk factors, and distal sensorimotor polyneuropathy: The KORA F4/FF4 studyPubmed:29577557
  • Selected elements of extracellular matrix of the skin in diabetes and insulin resistancePubmed: 31146169
  • Serum uromodulin is inversely associated with biomarkers of subclinical inflammation in the population-based KORA F4 study
  • Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort studyPubmed:34990470
  • EFFECTS OF HIS-PHE-ARG-TRP-PRO-GLY-PRO PEPTIDE ON FREE-RADICAL OXIDATION PROCESSES IN CONDITIONS OF CHRONIC RESTRAINT …

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