Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)Homo sapiens (Human)Polyclonal antibody

Neurotrophin; Abrineurin

  • Product No.PAA011Hu01
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig TypeIgG
  • PurificationAntigen-specific affinity chromatography
  • LabelNone
  • Immunogenn/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration200ug/ml
  • Organism Species Moren/a
  • ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
  • DownloadInstruction Manual
  • UOM10ug50ug100ug1mg1g
  • FOBUS$ 73US$ 169US$ 242US$ 968US$ 72600
  • Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)Package and Components
  • Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)Package and Components
  • Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)Used in DAB staining on fromalin fixed paraffin-embedded Heart tissue
  • PAA011Hu01.jpgWB Image
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Toxicological SciencesInteraction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat BrainsPubMed: 21436126
  • Journal of the Neurological SciencesNeuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cordScienceDirect: S0022510X12001219
  • International Conference on BioscienceEffect of Benthosemapterotum Peptide on Aging in Mice with Oxidative DamagePsrcentre: 212059
  • Neuroscience LettersChronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone miceScienceDirect: S0304394010015788
  • BioMed Research InternationalNeuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.NCBI: PMC3925622
  • Behavioural Brain ResearchThe effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factorPubmed:25446810