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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
Neurotrophin
Organism species Homo sapiens (Human)
Product No.PAA011Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant BDNF (His129~Arg247) expressed in E.coli.
Accession No.: RPA011Hu01
Sequence: The target protein is fused with a N-terminal GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GGPLGSPEF- HS DPARRGELSV CDSISEWVTA ADKKTAVDMS GGTVTVLEKV PVSKGQLKQY FYETKCNPMG YTKEGCRGID KRHWNSQCRT TQSYVRALTM DSKKRIGWRF IRIDTSCVCT LTIKRGR
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant BDNF (His129~Arg247) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA011Hu22Monoclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)WB, ICC, IHC-P, IHC-F, ELISA
PAA011Hu01Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)WB, ICC, IHC-P, IHC-F, ELISA
PAA011Hu71Biotin-Linked Antibody to Brain Derived Neurotrophic Factor (BDNF)IHC;WB;ELISA
PAA011Hu81FITC-Linked Antibody to Brain Derived Neurotrophic Factor (BDNF)IHC;ICC;IF;WB
RPA011Hu01Recombinant Brain Derived Neurotrophic Factor (BDNF)SDS-PAGE; WB; ELISA; IP.
SCA011HuCLIA Kit for Brain Derived Neurotrophic Factor (BDNF)Chemiluminescent immunoassay
SEA011HuELISA Kit for Brain Derived Neurotrophic Factor (BDNF)Enzyme-linked immunosorbent assay