KO-Validated
Polyclonal Antibody to Cystatin B (CSTB)

Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAJ322Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB;IHC;ICC/IF;FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryImmune molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPJ322Hu01-Recombinant Cystatin B (CSTB)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Thyroid Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CSTB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Esophagus cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human CSTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human MCF7 cell;
Primary Ab: 10µg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 1.3µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Figure. Western Blot; Sample: Recombinant CSTB, Human.
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: CSTB knockout HepG2 cell lysate;
Predicted MW: 11kDa
Observed MW: 14kDa
Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: MCF7 cell lysate; Lane3: A549 cell lysate;
Primary Ab: 0.05µg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human A431 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CSTB Polyclonal Antibody (Catalog PAJ322Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CSTB. It has been selected for its ability to recognize CSTB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
For flow cytometry: 0.5-2 µg per 10^6 cells in 100 µl volume.
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disordersPubMed: 23291538
Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancerPubMed: PMC3754495
Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal ApoptosisPubMed: PMC3365072
Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophagesPubmed:25209871
A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive systemScience: Article
Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.pubmed:28766361

KO-Validated Polyclonal Antibody to Cystatin B (CSTB)