Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)Homo sapiens (Human)Polyclonal antibody

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

  • Product No.PAH839Hu01
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig TypeIgG
  • PurificationAntigen-specific affinity chromatography
  • LabelNone
  • Immunogenn/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration200ug/ml
  • Organism Species Moren/a
  • ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
  • DownloadInstruction Manual
  • UOM10ug50ug100ug1mg1g
  • FOBUS$ 105US$ 245US$ 350US$ 1400US$ 105000
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)Package and Components
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)Package and Components
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
  • PAH839Hu01.jpgWB Image
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Molecular & Cellular ProteomicsIdentification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
  • Molecular & Cellular ProteomicsIdentification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
  • Clinical Cancer ResearchIsocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
  • Onco Targets TherThe effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546