• KO-Validated
Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Homo sapiens (Human) Polyclonal antibody

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) DAB staining on IHC-P;
    Samples: Human Liver Tissue
  • PAH839Hu01.jpg WB Image
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: IDH1 knockout HepG2 cell lysate;
    Predicted MW: 47kDa
    Observed MW: 47kDa
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Mouse Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Porcine Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Molecular & Cellular ProteomicsIdentification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
  • Molecular & Cellular ProteomicsIdentification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
  • Clinical Cancer ResearchIsocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
  • Onco Targets TherThe effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546

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