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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Keratin 10 (KRT10)
K10; CK10; KPP; Cytokeratin 10; Epidermolytic Hyperkeratosis; Keratosis Palmaris Et Plantaris
Organism species Homo sapiens (Human)
Product No.PAB691Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant KRT10 (Leu153~Glu456) expressed in E.coli.
Accession No.: RPB691Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- LNDRLASY LDKVRALEES NYELEGKIKE WYEKHGNSHQ GEPRDYSKYY KTIDDLKNQI LNLTTDNANI LLQIDNARLA ADDFRLKYEN EVALRQSVEA DINGLRRVLD ELTLTKADLE MQIESLTEEL AYLKKNHEEE MKDLRNVSTG DVNVEMNAAP GVDLTQLLNN MRSQYEQLAE QNRKDAEAWF NEKSKELTTE IDNNIEQISS YKSEITELRR NVQALEIELQ SQLALKQSLE ASLAETEGRY CVQLSQIQAQ ISALEEQLQQ IRAETECQNT EYQQLLDIKI RLENEIQTYR SLLEGE
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against KRT10. It has been selected for its ability to recognize KRT10 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant KRT10 (Leu153~Glu456) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB691Hu22Monoclonal Antibody to Keratin 10 (KRT10)WB, ICC, IHC-P, IHC-F, ELISA
PAB691Hu01Polyclonal Antibody to Keratin 10 (KRT10)WB, ICC, IHC-P, IHC-F, ELISA
RPB691Hu01Recombinant Keratin 10 (KRT10)SDS-PAGE; WB; ELISA; IP.
SEB691HuELISA Kit for Keratin 10 (KRT10)Enzyme-linked immunosorbent assay