Home  |   About us
ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Runt Related Transcription Factor 2 (RUNX2)
AML3; CBFA1; CCD; CCD1; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1
Organism species Homo sapiens (Human)
Product No.PAB011Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant RUNX2 (Tyr299~Tyr521) expressed in E.coli.
Accession No.: RPB011Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEFELRRQAC-YD QSYPSYLSQM TSPSIHSTTP LSSTRGTGLP AITDVPRRIS DDDTATSDFC LWPSTLSKKS QAGASELGPF SDPRQFPSIS SLTESRFSNP RMHYPATFTY TPPVTSGMSL GMSATTHYHT YLPPPYPGSS QSQSGPFQTS STPYLYYGTS SGSYQFPMVP GGDRSPSRML PPCTTTSNGS TLLNPNLPNQ NDGVDADGSH SSSPTVLNSS GRMDESVWRP Y
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against RUNX2. It has been selected for its ability to recognize RUNX2 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant RUNX2 (Tyr299~Tyr521) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
Download
Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB011Hu22Monoclonal Antibody to Runt Related Transcription Factor 2 (RUNX2)WB, ICC, IHC-P, IHC-F, ELISA
PAB011Hu01Polyclonal Antibody to Runt Related Transcription Factor 2 (RUNX2)WB, ICC, IHC-P, IHC-F, ELISA
RPB011Hu01Recombinant Runt Related Transcription Factor 2 (RUNX2)SDS-PAGE; WB; ELISA; IP.
SEB011HuELISA Kit for Runt Related Transcription Factor 2 (RUNX2)Enzyme-linked immunosorbent assay