Polyclonal Antibody to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)

LEKTI; LETKI; NETS; NS; VAKTI; Lymphoepithelial Kazal-Type-Related Inhibitor; Lympho-epithelial Kazal-type-related inhibitor
Western Blot

IMMUNOGEN INFORMATIONImmunogen: Recombinant SPINK5 (Gly699~Ser976) expressed in E.coli.
Accession No.: RPH145Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GG GNTQDECAEY REQMKNGRLS CTRESDPVRD ADGKSYNNQC TMCKAKLERE AERKNEYSRS RSNGTGSESG KDTCDEFRSQ MKNGKLICTR ESDPVRGPDG KTHGNKCTMC KEKLEREAAE KKKKEDEDRS NTGERSNTGE RSNDKEDLCR EFRSMQRNGK LICTRENNPV RGPYGKMHIN KCAMCQSIFD REANERKKKD EEKSSSKPSN NAKDECSEFR NYIRNNELIC PRENDPVHGA DGKFYTNKCY MCRAVFLTEA LERAKLQEKP SHVRAS
ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against SPINK5. It has been selected for its ability to recognize SPINK5 in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. QUALITY CONTROL Content: The quality control contains recombinant SPINK5 (Gly699~Ser976) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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    Polyclonal Antibody to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) Material Safety Data Sheet International Organization for Standardization guarantee