Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Homo sapiens (Human) Monoclonal antibody

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number 2#
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB081Hu01-Recombinant Heat Shock 70kDa Protein 1A (HSPA1A)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Packages (Simulation)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Mouse Anti-Human HSPA1A Antibody Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human HSPA1A Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human HSPA1A Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB081Hu21.jpg Figure. Western Blot; Sample: Recombinant HSPA1A, Human.
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Cavia Liver lysate; Lane3: Cavia Cerebrum lysate; Lane4: Canine Cerebrum lysate; Lane5: Bovine Liver lysate; Lane6: Bovine Cerebrum lysate
    Primary Ab: 1μg/ml Mouse Anti-Human HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Liver lysate; Lane3: Canine Liver lysate
    Primary Ab: 1μg/ml Mouse Anti-Human HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Jurkat cell lysate; Lane3: Rat Cerebrum lysate; Lane4: Rat Uterus lysate
    Primary Ab: 3µg/ml Mouse Anti-Human HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Heat Shock 70kDa Protein 1A (HSPA1A) Western Blot; Sample: Lane1: Bovine Liver lysate; Lane2: Bovine Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Hela cell lysate
    Primary Ab: 0.2µg/ml Mouse Anti-Human HSPA1A Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against HSPA1A. It has been selected for its ability to recognize HSPA1A in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Chicken (Gallus)

Giveaways

Citations

  • Identification of potential biomarkers of gold nanoparticle toxicity in rat brainsPubMed: 22691312
  • Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in PigsPubmed:Pmc4324980
  • Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in ratsPubmed:24508522
  • Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing micePubmed:25443743
  • A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmaticspubmed:28822267
  • Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …Pubmed:29379533
  • Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampusPubmed: 32172517
  • Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in micePubmed: 32439493

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