ELISA Kit for Anti-Mullerian Hormone (AMH) Chicken (Gallus) Sandwich ELISA

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

  • Product No.SEA228Ga
  • Organism SpeciesChicken (Gallus) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range15.6-1,000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 6.4pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH).No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 479.00 US$ 684.00 US$ 3,078.00 US$ 5,814.00 US$ 47,880.00
  • Quantity
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  • ELISA Kit for Anti-Mullerian Hormone (AMH) Packages (Simulation)
  • ELISA Kit for Anti-Mullerian Hormone (AMH) Packages (Simulation)
  • ELISA Kit for Anti-Mullerian Hormone (AMH) Results demonstration
  • SEA228Ga.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-Mullerian Hormone (AMH) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Mullerian Hormone (AMH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-96 90
EDTA plasma(n=5) 93-105 98
heparin plasma(n=5) 82-89 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Mullerian Hormone (AMH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 95-103% 83-93% 91-105%
EDTA plasma(n=5) 84-92% 83-102% 82-98% 78-88%
heparin plasma(n=5) 98-105% 96-105% 88-101% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Anti-Mullerian Hormone (AMH)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Mullerian Hormone (AMH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anti-Mullerian Hormone (AMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Mullerian Hormone (AMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mullerian Hormone (AMH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Journal of Zhejiang University SCIENCE BInfluence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice.PubMed: 23125082
  • In Vivo.(Athens,Greece)Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVFPubmed: 24292596
  • Gynecological EndocrinologyThe effects of isotretinoin on the ovarian reserve of females with acneInformahealthcare: Source
  • Fertility and SterilityIncreased expression of anti mullerian hormone and its receptor in endometriosisIngentaconnect: Source
  • Reproductive BioMedicine OnlineDecreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposureRbmojournal: Source
  • Reproductive Biology and EndocrinologyThe influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study.Pubmed: 24341292
  • Biomed Res Int.Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in RatsPubmed:Pmc4053297
  • Fertil Steril. Increased expression of antimüllerian hormone and its receptor in endometriosisPubmed:24613539
  • The Journal of Maternal-Fetal &amp; Neonatal MedicineMaternal serum anti-Müllerian hormone at 11-13 weeks&#039; gestation in the prediction of preeclampsiaPubmed:24953352
  • J Gynecol Oncol.Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat modelPubmed:25142624
  • Reproductive BioMedicine OnlineThe effect of childhood cancer therapy on ovarian reserve and pubertal developmentPubmed:25498597
  • Reproductive SciencesEffects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary SyndromePubmed:25667201
  • Yonsei Medical JournalAnti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell LinesPubMed: 26632380
  • oncology researchMechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in RatsPubMed: 26629940
  • Reprod Biomed OnlineProtective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley ratsPubMed: 26206282
  • PLoS OneGrowth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R AxisPubMed: 26465611
  • Toxicol LettLong-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthoodPubMed: 26657179
  • Journal of Assisted Reproduction and GeneticsCan Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage?Pubmed:27255570
  • Anticancer Res.Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast CancerPubmed:26976997
  • Chemosphere. Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring.pubmed:27567155
  • TheriogenologyAge-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males.pubmed:28043339
  • environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.pubmed:27838061
  • Journal of Assisted Reproduction and GeneticsIntrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis.pubmed:28074436
  • Journal Pakistan Medical AssociationSerum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy micepubmed:27654725
  • Advance Medical AssociatesAre anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes?pubmed:27162065
  • Yonsei Med J VolumeAnti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines.pubmed:26632380
  • Journal of Endocrinological InvestigationThe use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF.pubmed:27465668

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