ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1) Mus musculus (Mouse) Sandwich ELISA

Endocan

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  • ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1) Packages (Simulation)
  • ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1) Packages (Simulation)
  • ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1) Results demonstration
  • SEC463Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Endothelial Cell Specific Molecule 1 (ESM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelial Cell Specific Molecule 1 (ESM1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-103 98
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 84-98 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelial Cell Specific Molecule 1 (ESM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelial Cell Specific Molecule 1 (ESM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelial Cell Specific Molecule 1 (ESM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 83-98% 80-93% 80-95%
EDTA plasma(n=5) 85-97% 94-102% 87-98% 88-97%
heparin plasma(n=5) 85-101% 98-105% 94-105% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Endothelial Cell Specific Molecule 1 (ESM1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial Cell Specific Molecule 1 (ESM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelial Cell Specific Molecule 1 (ESM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantationPubMed: 22608803
  • Endocan is useful biomarker of survival and severity in sepsis.Pubmed: 24769132
  • Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromesPubmed:25211383
  • Endocan is useful biomarker of survival and severity in sepsisPubmed:24769132
  • Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus ErythematosusPubMed: 26614790
  • Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control studyJournal:-102
  • Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension.pubmed:27912147
  • Diagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancerpubmed:28514746
  • Changes in Endocan Levels and Blood Coagulation in HIV InfectionDOI: 10.13005/bpj/1082
  • A novel role of endocan in alleviating LPS-induced acute lung injuryPubmed:29627442
  • Is the plasma endocan level a reliable predictor for the severity of erectile dysfunction?Pubmed:30054863
  • Serum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease ActivityDoi: 10.2174/1874312901812010189
  • Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activityPubmed: 30816462
  • Endothelial biomarkers in the light of new sepsis definitionPubmed: 30920847
  • Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …
  • A novel marker relationship between carotid intima–media thickness and disease activity score-28 in patients with rheumatoid arthritis: human endothelial cell …Pubmed: 31655533
  • Role of Ascitic Endocan Levels in the Diagnosis of Spontaneous Bacterial Peritonitis in Decompensated CirrhosisPubmed: 32364003
  • Comparison of the maternal serum endocan levels in preterm premature rupture of membrane and normal pregnancy34109715
  • The relationship between serum endocan levels with the presence of contrast-induced nephropathy in patients undergoing coronary angiography

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