ELISA Kit for Angiotensinogen (AGT)

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
Typical Standard Curve Typical Standard Curve

Sample type Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluid. Detection range 1.563-100ng/mL The standard curve concentrations used for the ELISA's were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.72ng/mL. Specificity This assay has high sensitivity and excellent specificity for detection of Angiotensinogen (AGT).
No significant cross-reactivity or interference between Angiotensinogen (AGT) and analogues was observed.

Recovery Matrices listed below were spiked with certain level of recombinant Angiotensinogen (AGT) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensinogen (AGT) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)88-9994
EDTA plasma(n=5)88-9592
heparin plasma(n=5)87-10398

Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensinogen (AGT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)93-102%93-101%98-105%82-104%
EDTA plasma(n=5)95-103%82-93%92-101%97-105%
heparin plasma(n=5)84-98%88-96%79-102%98-105%

Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensinogen (AGT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensinogen (AGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensinogen (AGT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensinogen (AGT) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Assay procedure summary 1. Prepare all reagents, samples and standards;
Reagent Preparation
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Reference
  • Transplant InternationalRead more(Wiley: source)Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients?
  • AmyloidRead more(PubMed: 22320202)The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients
  • Pediatric MephrologyRead more(PubMed: PMC3661928)Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension
  • Acta PaediatricaRead more(Pubmed: 23772991)Urinary angiotensinogen as a novel marker of obstructive nephropathy in children
  • Journal of the Renin-Angiotensin-Aldosterone SystemRead more(Sagepub: Source)Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia
  • Pediatric NephrologyRead more(Oxfordjournals:Source)MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY
  • Journal of Renin-Angiotensin-Aldosterone SystemRead more(Pubmed:24532824)Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia
Reagents and materials provided
  • Pre-coated, ready to use 96-well strip plate1
  • Plate sealer for 96 wells4
  • Standard2
  • Standard Diluent1×20mL
  • Detection Reagent A1×120µL
  • Assay Diluent A1×12mL
  • Detection Reagent B1×120µL
  • Assay Diluent B1×12mL
  • TMB Substrate1×9mL
  • Stop Solution1×6mL
  • Wash Buffer (30 × concentrate)1×20mL
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