ELISA Kit for Anterior Gradient 2 (AGR2) Homo sapiens (Human) Sandwich ELISA

GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Anterior Gradient 2 (AGR2) Packages (Simulation)
  • ELISA Kit for Anterior Gradient 2 (AGR2) Packages (Simulation)
  • ELISA Kit for Anterior Gradient 2 (AGR2) Results demonstration
  • SEC285Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Anterior Gradient 2 (AGR2) and the recovery rates were calculated by comparing the measured value to the expected amount of Anterior Gradient 2 (AGR2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-96 89
EDTA plasma(n=5) 86-102 98
heparin plasma(n=5) 86-95 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anterior Gradient 2 (AGR2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 84-93% 94-105% 98-105%
EDTA plasma(n=5) 90-99% 92-104% 96-105% 80-103%
heparin plasma(n=5) 83-104% 80-89% 78-90% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Anterior Gradient 2 (AGR2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient 2 (AGR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anterior Gradient 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anterior Gradient 2 (AGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anterior Gradient 2 (AGR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinomaMetaPress: n7137r6271u54118
  • Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer BiomarkersPubMed: PMC3205865
  • Anterior gradient 2 (AGR2): blood-based biomarker elevated in metastatic prostate cancer associated with the neuroendocrine phenotypePubmed: 22911164
  • Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.9Pubmed: 24007603
  • Validation of Biomarkers That Complement CA19.9 in Detecting Early Pancreatic CancerPubmed:25239611
  • AGR3 in Breast Cancer: Prognostic Impact and Suitable Serum-Based Biomarker for Early Cancer DetectionPubMed: 25875093
  • Serum AGR2 as a useful biomarker for pituitary adenomasS0303846717300045
  • Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2)Pubmed:29937991
  • Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy‑induced senescence escape34913074
  • Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancerPubmed:35689436

Recommend products