ELISA Kit for Alpha-Fetoprotein (aFP) Homo sapiens (Human) Sandwich ELISA

  • Promotion products

A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein

  • Product No.SEA153Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.78-50ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.26ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP).No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 Ensure in stock; if not, free redo!
  • FOB US$ 349.00 US$ 498.00 US$ 2,241.00 US$ 4,233.00 US$ 34,860.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Alpha-Fetoprotein (aFP) Packages (Simulation)
  • ELISA Kit for Alpha-Fetoprotein (aFP) Packages (Simulation)
  • ELISA Kit for Alpha-Fetoprotein (aFP) Results demonstration
  • SEA153Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Alpha-Fetoprotein (aFP) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-Fetoprotein (aFP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-94 83
EDTA plasma(n=5) 89-103 95
heparin plasma(n=5) 80-98 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (aFP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (aFP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-Fetoprotein (aFP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 89-103% 94-102% 78-89%
EDTA plasma(n=5) 81-91% 98-105% 78-88% 90-99%
heparin plasma(n=5) 92-105% 83-94% 82-95% 88-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Alpha-Fetoprotein (aFP)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (aFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (aFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (aFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Fetoprotein (aFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • BioFactorsGinger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis modelPubMed: 20872761
  • Food and Nutrition SciencesInvestigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda.Scirp: 5684
  • Diabetes Research and Clinical PracticePolyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic ratsScienceDirect: S0168822711000520
  • Life SciencesComparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liverScienceDirect: S0024320511002700
  • Annals of HepatologyCytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes.Hepatology: source
  • Biomedicine & Aging PathologyChemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar ratsScienceDirect: S2210522013000348
  • European journal of pharmacologySuramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzymePubmed: 24530413
  • Asian Pac J Cancer Prev.Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention.Pubmed:25684529
  • Journal of Analytical Atomic SpectrometryDetection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometryRsc:Source
  • PLOS ONEDevelopment of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular CarcinomaPubmed:24927126
  • BMC Med ImagingEvaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinomaPubMed: 25981587
  • Tumour BiolElevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinomaPubMed: 26643893
  • Acta Veterinaria BrnoThe concentrations of selected blood serum proteins in calves during the first three months of life85:33
  • Hepatobiliary Pancreat Dis IntPredictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion patternabstract:abstract4525.shtml
  • Journal of Applied Pharmaceutical ScienceChemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats2071_pdf.pdf

Recommend products