ELISA Kit for Hepatocyte Growth Factor (HGF) Homo sapiens (Human) Sandwich ELISA

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

  • Product No.SEA047Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range31.2-2,000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 12.3pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 Ensure in stock; if not, free redo!
  • FOB US$ 319.00 US$ 455.00 US$ 2,048.00 US$ 3,868.00 US$ 31,850.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Hepatocyte Growth Factor (HGF) Packages (Simulation)
  • ELISA Kit for Hepatocyte Growth Factor (HGF) Packages (Simulation)
  • ELISA Kit for Hepatocyte Growth Factor (HGF) Results demonstration
  • SEA047Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Hepatocyte Growth Factor (HGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Hepatocyte Growth Factor (HGF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-94 91
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 93-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepatocyte Growth Factor (HGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 80-94% 85-103% 96-103%
EDTA plasma(n=5) 78-97% 94-103% 80-90% 83-94%
heparin plasma(n=5) 90-103% 96-103% 87-103% 91-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Hepatocyte Growth Factor (HGF)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor (HGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor (HGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor (HGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor (HGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Gene Therapy and Molecular BiologySphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heartLWW: 01000
  • Journal of Translational MedicineLow temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinomaBioMed: 14795876
  • Digestive Diseases and SciencesGene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver FailureSpringerLink: k22823l138x2344x
  • Biomarker insightsCorrelation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic ChildrenPubMed: PMC3694825
  • Biomarker insightsDecreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD)PubMed: PMC3762604
  • BMC cell biologyBMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
  • Cell Journal (Yakhteh)Effect of Mesenchymal Stem Cells on Doxorubicin-Induced FibrosisPubMed: PMC3584430
  • Gastroenterol Res PractProinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver CirrhosisPubMed: 26448742
  • Tumor BiologyHypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • CytotherapyCell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injuryPubMed: 25863963
  • Biomedicine & PharmacotherapySilymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated ratsPubmed:27261582
  • Can J Physiol Pharmacol. Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosisPubmed:27186801
  • Genet Mol ResChanges in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  •  Changes in HGF levels after a progressive test before and after Tabata HIIT programprofile:Pawel_Wolanski3

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