ELISA Kit for Laminin Alpha 5 (LAMa5)
LAM-A5
- UOM
- FOB US$ 490.00 US$ 700.00 US$ 3,150.00 US$ 5,950.00 US$ 49,000.00
- Quantity
Overview
Properties
- Product No.SEC078Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryDevelopmental science
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Recovery
Matrices listed below were spiked with certain level of recombinant Laminin Alpha 5 (LAMa5) and the recovery rates were calculated by comparing the measured value to the expected amount of Laminin Alpha 5 (LAMa5) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-101 | 93 |
EDTA plasma(n=5) | 91-98 | 95 |
heparin plasma(n=5) | 94-102 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Laminin Alpha 5 (LAMa5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 78-101% | 86-102% | 79-104% | 86-96% |
EDTA plasma(n=5) | 81-103% | 87-94% | 90-98% | 96-104% |
heparin plasma(n=5) | 95-102% | 87-105% | 83-95% | 91-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 5 (LAMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 5 (LAMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 5 (LAMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 5 (LAMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosisPubmed: 24188612
- SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological materialPubmed:27209594
- Superoxide Dismutase-3 Downregulates Laminin α5 Expression in Tumor Endothelial Cells via the Inhibition of Nuclear Factor Kappa B SignalingPubmed:35267534