ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)Homo sapiens (Human)Sandwich ELISA

PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2

  • Product No.SEA699Hu
  • Organism SpeciesHomo sapiens (Human)
  • Sample Typeserum, plasma, tissue homogenates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.312-20ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.129ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2).
    No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 441US$ 630US$ 2835US$ 5355US$ 44100
  • ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)Package and Components
  • ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)Package and Components
  • ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)Results demonstration
  • SEA699Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Prostaglandin Endoperoxide Synthase 2 (PTGS2) and the recovery rates were calculated by comparing the measured value to the expected amount of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)95-104101
EDTA plasma(n=5)80-9285
heparin plasma(n=5)93-10196

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)89-97%81-96%93-101%98-105%
EDTA plasma(n=5)89-103%79-103%90-97%84-99%
heparin plasma(n=5)78-91%92-102%82-101%86-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

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  • Evidence-Based Complementary and Alternative MedicineCurcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in CarcinogenesisPubMed: 22888366
  • Integrative Cancer TherapiesDecalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunitsPubmed: 24013642
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  • Int J Clin Exp PatholRelationship between epidermal growth factor receptor (EGFR) mutation and serum cyclooxygenase-2 Level, and the synergistic effect of celecoxib and gefitinib on EGFR expression in non-small cell lung cancer cellsPubMed: 26464643
  • Int J Clin Exp PatholMonitoring of cyclooxygenase-2 levels can predict EGFR mutations and the efficacy of EGFR-TKI in patients with lung adenocarcinomaPubMed: 26191267