ELISA Kit for Renalase (RNLS) Homo sapiens (Human) Sandwich ELISA

MAO-C; C10orf59; FAD-Dependent Amine Oxidase; Monoamine oxidase-C; alpha-NAD(P)H oxidase/anomerase

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  • ELISA Kit for Renalase (RNLS) Packages (Simulation)
  • ELISA Kit for Renalase (RNLS) Packages (Simulation)
  • ELISA Kit for Renalase (RNLS) Results demonstration
  • SEC845Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Renalase (RNLS) and the recovery rates were calculated by comparing the measured value to the expected amount of Renalase (RNLS) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-93 88
EDTA plasma(n=5) 78-101 97
heparin plasma(n=5) 82-96 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renalase (RNLS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renalase (RNLS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Renalase (RNLS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-99% 95-103% 81-97% 85-99%
EDTA plasma(n=5) 95-104% 87-95% 88-101% 80-101%
heparin plasma(n=5) 79-90% 78-95% 92-101% 78-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Renalase (RNLS)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renalase (RNLS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renalase (RNLS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renalase (RNLS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Renalase (RNLS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Renalase, kidney function, and markers of endothelial dysfunction in renal transplant recipientsCartelcolor: source
  • Renalase, Stroke, and Hypertension in Hemodialyzed PatientsPubMed: 22583169
  • Renalase in peritoneal dialysis patients is not related to blood pressure, but to dialysis vintage.Pdiconnect: Source
  • Vascular adhesion protein-1 and renalase in regard to diabetes in hemodialysis patientsPubMed: PMC3542495
  • Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis PatientsPubmed: 23530612
  • VAP-1 in chronic kidney disease and diabetic kidney diseaseCzytelniamedyczna: Source
  • The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathyPubmed: 15365253
  • Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients.Pubmed: 24599883
  • Renalase, a novel enzyme involved in blood pressure regulation, is related to kidney function but not to blood pressure in hemodialysis patients.Pubmed: 22539018
  • Renalase in hypertension and kidney diseaseOxfordjournals: Source
  • Markers of increased cardiovascular risk in patients with chronic kidney diseaseBiomedcentral:Source
  • Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipientsPubmed:24599883
  • Normal values for urine renalase excretion in childrenPubmed:25060760
  • Low plasma renalase concentration in hypertensive patients after surgical repair of coarctation of aortaPubmed:25064768
  • Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adultsPubmed:25824645
  • Circulating renalase, catecholamines, and vascular adhesionPubMed: 26403854
  • Renalase levels in children with solitary functioning kidneyPubMed: 26713989
  • SP321 RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTSContent: 30
  • PP.27.07: PREHYPERTENSIVE ALTERATIONS OF ARTERIAL AND VENOUS REACTIVITY IN A RAT POLYCYSTIC OVARY SYNDROME MODEL. EFFECTS OF PARALLEL VITAMIN D3 ADMINISTRATIONJhypertension: Abstract
  • PP.27.06: RENALASE, RENAL FUNCTION AND BIOMARKERS OF ENDOTHELIAL DYSFUNCTION IN CHRONIC KIDNEY DISEASE PATIENTS.Jhypertension: Abstract
  • PP.27.04: ENDOTHELIAL DYSFUNCTION AND ARTERIAL HYPERTENSION IN THE WORKING POPULATION (MERIDIAN STUDY).Jhypertension: Abstract
  • Renalase is Removed by Kidneys and During Dialysis – Excess Related to CKD Complications?PubMed: 25515790
  • Effects of Renin-Angiotensin System Inhibitors on Renal Expression of Renalase in Sprague-Dawley Rats Fed With High Salt DietPubMed: 26619289
  • Serum Renalase Levels Correlate with Disease Activity in Lupus NephritisPubMed: 26431044
  • Plasma renalase concentration before and after radiofrequency renal denervation in patients with resistant hypertensionPubMed: 26310184
  • Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography.pubmed:27778420
  • PLASMA RENALASE CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION: PRELIMINARY RESULTScontent:31
  • Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy?pubmed:27416751
  • High-performance Liquid Chromatography Measured Metabolites of EndogenousCatecholamines and Their Relations to Chronic Kidney Disease and High Blood Pressure in Heart Transplant Recipients.pubmed:27496485
  • Online Haemodiafiltration Improves Inflammatory State in Dialysis Patients: A Longitudinal Study.pubmed:27783636
  • Renalase in Children with Glomerular Kidney Diseaseschapter:10.1007
  • Plasma Renalase is Not Associated with Blood Pressure and Brachial-Ankle Pulse Wave Velocity in Chinese Adults With Normal Renal Function452587
  • Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controlspubmed:28372594
  • Increased serum renalase in peritoneal dialysis patients: Is it related to cardiovascular disease riskpubmed:28160962
  • Age influence on renalase and catecholamines concentration in hypertensive patients, including maintained dialysispubmed:27822026
  • Aumento de renalasa sérica en pacientes de diálisis peritoneal:¿ está relacionado con el riesgo de enfermedad cardiovascular?10.1016:j.nefro.2016.11.013
  • Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patientspubmed:28614373
  • Levels of renalase and advanced oxidation protein products with regard to catecholamines in haemodialysed patientspubmed:28954489
  • Endocan Concentration in Patients With Primary Hypertensionpubmed:29084442
  • RENALASE PLASMA CONCENTRATION BEFORE AND AFTER RADIOFREQUENCY RENAL DENERVATION IN PATIENTS WITH RESISTANT HYPERTENSION10.1097/01.hjh.0000523893.07419.ae
  • Renalase Protects against Renal Fibrosis by Inhibiting the Activation of the ERK Signaling Pathwayspubmed:28448446
  • Transient changes in serum renalase concentration during long-distance running: The case of an amateur runner under continuous training10.7600/jpfsm.6.159
  • Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease‒a cross-sectional studyPubmed:29743680
  • Influence of acute exercise on renalase and its regulatory mechanismPubmed:30056018
  • Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degenerationPubmed:29580721
  • A new method for quantitative determination of renalase based on mass‐spectrometry determination of a proteotypic peptide labeled with stable isotopes10.1002:rcm.8167
  • Renalase contributes to protection against renal fibrosis via inhibiting oxidative stress in ratsPubmed:29476431
  • Serum Renalase Levels in Adolescents with Primary HypertensionPubmed:29748702
  • Atorvastatin facilitates protection against contrast-induced nephropathy in patients undergoing coronary angiography via humoral mediators rather than …Pubmed:30008773
  • High urinary kidney injury molecule type 1 level is associated with 12-month adverse cardiovascular events in patients with coronary artery diseasepublication:324522368
  • Serum Renalase Levels Are Predicted by Brain-Derived Neurotrophic Factor and Associated with Cardiovascular Events and Mortality after Percutaneous Coronary …Pubmed: 30424498
  • GP23 Percutaneous closure of patent ductus arteriosus: single center experience
  • GP25 Comparison of associations and outcomes of prenatally and postnatally diagnosed right aortic arch: south wales' experience
  • GP24 Serum renalase levels in adolescents with primary hypertension
  • Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot StudyPubmed: 31737132
  • The role of urinary renalase on early-stage renal damage in Chinese adults with primary hypertensionPubmed: 32102561
  • Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of ratsPubmed: 32053739
  • The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patientsPubmed: 32297799
  • Association of Serum Renalase Levels and Renalase rs10887800 Polymorphism with Unstable Angina Pectoris Patients Having Metabolic SyndromePubmed: 32982355
  • Nikotinamidadenindinukleotid-Blutplasmawerte sind unabhängig vom kalendarischen und vaskulären Alter in einer Normalkohorte aus der Region Halle-Saalekreis
  • Circulating Renalase as Predictor of Renal and Cardiovascular Outcomes in Pre-Dialysis CKD Patients: A 5-Year Prospective Cohort Study33800219
  • Edited by: Background: Renalase has been implicated in chronic heart failure (CHF); however, nothing is known about renalase discriminatory ability and prognostic …

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