ELISA Kit for S100 Calcium Binding Protein A7 (S100A7) Bos taurus; Bovine (Cattle) Sandwich ELISA

S100-A7; PSOR1; S100A7c; Psoriasin 1

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  • ELISA Kit for S100 Calcium Binding Protein A7 (S100A7) Packages (Simulation)
  • ELISA Kit for S100 Calcium Binding Protein A7 (S100A7) Packages (Simulation)
  • ELISA Kit for S100 Calcium Binding Protein A7 (S100A7) Results demonstration
  • SEC035Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant S100 Calcium Binding Protein A7 (S100A7) and the recovery rates were calculated by comparing the measured value to the expected amount of S100 Calcium Binding Protein A7 (S100A7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 95
EDTA plasma(n=5) 84-98 89
heparin plasma(n=5) 80-91 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A7 (S100A7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A7 (S100A7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100 Calcium Binding Protein A7 (S100A7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 82-101% 89-96% 85-92%
EDTA plasma(n=5) 90-97% 93-105% 88-95% 91-101%
heparin plasma(n=5) 88-96% 92-101% 88-103% 91-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for S100 Calcium Binding Protein A7 (S100A7)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A7 (S100A7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A7 (S100A7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A7 (S100A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A7 (S100A7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid estersSpringerLink: t07u24t43r357l15
  • Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS ApproachPubMed: 25807139
  • S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signalingPubmed:27060579
  • S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signallingpubmed:27060579
  • Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescriptionpubmed:28672924
  • The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function.pubmed:29142248
  • A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosisPubmed:28681537
  • Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping techniquePubmed:29374283
  • Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative statePubmed:30019218
  • Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detectionPubmed: 31706945
  • Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris

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