Mouse Model for Alcoholic Hepatitis (AH) Mus musculus (Mouse) Disease model

Alcoholic Liver Disease

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceAcute alcoholic liver disease(ALD) model induced by alcohol intake
  • Model Animal StrainsKM Mice (SPF), healthy ,male, bodyweight:18g~22g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group (Tiopronin) and Test drug group (three doses)
  • Modeling Period15d
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  • Mouse Model for Alcoholic Hepatitis (AH) Packages (Simulation)
  • Mouse Model for Alcoholic Hepatitis (AH) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. Except for normal group, the other groups were in the morning with 50% ethanol by 12ml/kg gavage 1 times, continuous 15d, at the same time, the daily afternoon drug group mice were divided into low, middle and high dose administered to give the corresponding drugs, positive control group 30mg/kg treated with Tiopronin suspension, were 1 times a day, in normal model group was given the same volume of distilled water.
2. After the last irrigation, fasting and free drinking water 16h, pick the eye for blood and extract the serum, into the -80 degree for storage. The liver was removed and the mass was calculated. At the same time, part of the left lobe of the liver in 10% neutral formalin solution for pathological examination, a portion of the left lobe of the liver in liquid nitrogen frozen for molecular biological examination.

Model evaluation

1. Liver coefficient: liver coefficient = organ wet mass / body mass.
2. Determination of blood biochemical parameters: the level of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and triglyceride (TG) were measured by automatic biochemical analyzer.

Pathological results

The pathological changes of liver: HE staining showed that there was no obvious fatty degeneration in the liver of the normal group, and the hepatic lobule structure was normal. In the model group, the liver was filled with a lot of fat degeneration.

Cytokines level

The contents of TG, TC, MDA, GSH in liver tissue were detected.

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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