Mouse Model for Hepatitis Mus musculus (Mouse) Disease model

Add to Cart Distributors
Overview
Properties
  • Prototype SpeciesHuman
  • SourceConA-induced Hepatitis
  • Model Animal StrainsBalb/c mice (SPF level), male, week age:8w-10w, body weight:30g~35g
  • Modeling GroupingRandomly divided into 6 groups: Control group, Model group, Positive drug group and Test drug group(three doses), 15 mice per group.
  • Modeling Period6 hours
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Mouse Model for Hepatitis Packages (Simulation)
  • Mouse Model for Hepatitis Packages (Simulation)
  • Mouse Model for Hepatitis Fig. HE staining, Liver of control group mice, Liver cells show radioactive arrangement in central veins.
  • DSI536Mu01.png Fig. HE staining, liver of Model group.
  • Mouse Model for Hepatitis Fig. The livers of modeling mice
  • Mouse Model for Hepatitis Fig. HE staining of livers
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

ConA dissolved in 0.01 mol/L phosphate buffered saline (PBS), with a concentration of 4mg/ml, with the dose of 20mg/kg from mice tail vein injection, 6h after injection, mice are sacrificed. At the same time, for the control group mice, inject PBS. All mice are free feeding and drinking water.

Model evaluation

1.Assay for ALT, AST, MPO
Pick eyeball to take blood at 6hrs after modeling,3000r/min, 4℃,10mins, collect the upper serum, stored at -80℃. Compared with the control group, the serum Alanine aminotransferase(ALT), aspartate aminotransferase (AST) and Myeloperoxidase (MPO) are significantly increased in the model group.

Pathological results

Take a liver lobe, with 4% poly formaldehyde solution fixed, conventional paraffin embedded sections, observation of pathological changes. In the control group, the structure of the hepatic lobe is complete, and the hepatic cells are centered in the central vein. In model group, hepatic cell cord arranged in disorder, liver sinus congestion is a common, hepatic lobule most liver cell swelling, fibrous septa formation, spotty necrosis and focal necrosis. A large number of neutrophils, infiltration of lymphocytes and mononuclear cells, extensive hepatocyte addicted to acid degeneration, vacuolar degeneration and fatty change can be observed in the focal necrosis.

Cytokines level

After the liver injury, the expression of TNFα, IFNγ, IL-2, IL-6, ICAM1 and other cytokines in serum is significantly increased, which could be detected by ELISA method.

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

Recommend products